This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
Agar is so critical that since WWII, scientists have tried to find alternatives in the event of a supply chain breakdown, especially as recent shortages have caused similar alarm. But while other colloid jellies have emerged, agar remains integral to laboratory protocols because no alternatives can yet compete on performance, cost, and ease of use.。关于这个话题，WPS官方版本下载提供了深入分析
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